My name is Hirobe Ryoma. I am twenty two years old. I live in my parents’ house in Fukui. There are five people in my family and I have my younger brother and younger sister. I have a car and I go to college by the car. I work part-time three days a week at convenience store and I have worked for four years. Because I have been working for a long time, I am acquaintances with many customers and spoken by them. I belonged Japanese archery club when I was a university student. I started doing Japanese archery when I was high school student. To play Japanese archery is very interesting and became mental training. If I have the opportunity I want to do Japanese archery again but now I have no time. I am studying production of useful substances of cells. My research theme is to cultivate using nutrient reduced medium, thereby aiming at low cost and efficient culture. If the nutrients are excessive in the medium, the cells will use a lot of nutrients. On the other hand, if the nutrients in the medium are low, the cells can’t live. So I am researching the culture method that cells don’t excessively consume nutrients and cells can live.
Outline of experiment
Biologics production using cell culture technology includes production of antibodies used for experiments and disease diagnosis and biopharmaceuticals used for treatment of many diseases. However, expensive culture equipment and culture media are used for cell culture, so research and production tend to be expensive. In the first place cells are excessive in nutrients In the beginning of the culture wasteful metabolism is consumed more than necessary nutrients, when the environment of nutrients in the latter stage of cultivation becomes too little survival rate and the production rate of useful substances such as antibodies drops . Especially in a commercially available medium, since nutrient composition is efficient with high efficiency throughout the whole culture, nutrients are excessively present at the initial stage of culture. So last year I decided to dilute the medium with phosphate buffer (PBS) to reduce the nutrients in the medium, thereby reducing the waste of nutrients. As a result, it was found that the antibody production efficiency per nutrient was more than doubled when the medium concentration was diluted to 50% as compared with the culture using ordinary medium. On the other hand, since the nutrients are reduced, the survival rate is low in the later stage of culture and it is unsuitable for long-term culture. Therefore, this year we are planning to solve the problem of survival rate and to develop a more efficient culturing method for a long time. I am doing now, replenish the nutrients by adding the media in the middle of the cell culture, whereby we believe that trying to improve the survival rate. Although this method could improve the survival rate, there is a problem that the cell density does not increase too much. Therefore, we need to try other improvement methods. We are investigating methods of culturing cells by adding factors to protect them from stress. In this method, we think that the problem of the previous experiment is improved, and the cell density will increase.
HomeAbou In recent years, Japanese large corporations are exposed to management difficulties, and there are cases in which bankruptcy is forced. Also, the growth rate of Japan's GDP has slowed down. In view of this situation, we believe that establishing a new industry of the next generation and promoting Japanese economic growth and metabolism of industry is important for the current situation where the declining birthrate and aging population progresses. Therefore, we think that it is desirable to form a social climate that supports such creation of venture companies.We should increase the supply of risk money in Japan. In Japan it is often said that it will be impossible to reoccur if you fail to start a business, it is said to be tough to fail. Therefore, the amount of supply of risk money is about 10% compared with the United States, and supply amount is small. It is difficult for new projects to be promoted. In fact, information-based start-up companies actually have a major impact on the creation of employment and GDP in the United States.People who graduated from an excellent university in Japan should start up their business. Excellent people in Japan tend to go to a low-risk, stable road such as a large company. While this is important to run a stable society, it is considered less contributing to the economy long-term development of. This is because, for the science and technology continue to progress, the existing companies because hard to create innovative outcomes. Meanwhile, in the United States, the more excellent people, the higher the tendency to aim for entrepreneurs and cultural differences arise. It seems that Japanese people lack frontier spirit like this.Despite having the largest economic infrastructure like the United States, there is a virtuous circle of expanding the economic scale further by the birth of new companies one after another. It is thought that in Japan, you should emulate this system and promote the creation of new industries and the metabolism of the existing social infrastructure.tDONATE